Review



2017 pcmv abemax koblan  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Addgene inc 2017 pcmv abemax koblan
    2017 Pcmv Abemax Koblan, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2017+pcmv+abemax+koblan/pCMV_ABEmax+(Plasmid+%23112095)/pm36206740-308-91-97
    Average 94 stars, based on 74 article reviews
    2017 pcmv abemax koblan - by Bioz Stars, 2026-11
    94/100 stars

    Images

    Related Articles

    Sequencing:

    Article Title: Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RIPA buffer Santa Cruz Cat#sc-24948 Intercept blocking buffer (TBS-based) LI-COR Cat#927-60001 PVDF membranes LI-COR Cat#926-31097 Protease inhibitor cocktail tablet Roche Cat#05892970001 TALON cobalt affinity resin Takara Cat#635504 CNBr-activated Sepharose 4B GE Healthcare Cat#17043001 Cycloheximide Sigma Cat#C7698 Oligomycin Cayman Cat#11342 Rotenone Cayman Cat#13995 Antimycin A Sigma Cat#A8674 Ionomycin Cayman Cat#11932 Carbachol Cayman Cat#114486 45Ca2+ radioisotope Perkin-Elmer Cat#NEZ013001MC 35S-methionine Perkin-Elmer Cat#NEG009A001MC Cysteine/methionine free medium Sigma Cat#D0422 Protein A beads Santa Cruz Cat#sc-2001 Thapsigargin Cayman Cat#10522 Digitonin Sigma-Aldrich Cat#D141 Calcium green 5N Life Technologies Cat#C3737 TMRM Invitrogen Cat#T668 FCCP Cayman Cat#15218 Doxorubicin Tokyo Chemical Industry Cat#D4193 Cisplatin Tokyo Chemical Industry Cat#D3371 Annexin V CF488A Biotium Cat#29083 Propidium Iodide Cayman Cat#14289 Hoescht 33,258 Cayman Cat#16756 Luciferin ThermoFisher Cat#PI88291 Critical commercial assays Seahorse XF Real-time ATP Rate Assay Kit Agilent Cat#103591-100 QuickChange II Kit Agilent Cat#200524 BCA assay kit Thermo Cat#23225 NativePAGE Sample Prep kit Invitrogen Cat#BN2008 NativePAGE Running Buffer kit Invitrogen Cat#BN2007 Deposited data Unprocessed images used in the figures This paper https://doi.org/10.17632/shrtrdfhyt.1 Experimental models: Cell lines HEK 293T: wild type ATCC Cat#CRL-11268; RRID: CVCL_1926 HeLa: wild type ATCC Cat#CCL-2; RRID: CVCL_0030 U2OS: wild type Cell Technologies Resource Center in the University of Colorado Anschutz Medical Campus https://medschool.cuanschutz.edu/ colorado-cancer-center/research/ shared-resources/cell-technologies/ cell-culture-services HEK 293T: MICU1-KO Home made Tsai et al. (2017) HEK 293T: MICU2-KO Home made Fan et al. (2020) HEK 293T: MICU1-MICU2-KO Home made Fan et al. (2020) HEK 239T: C463R-MICU1 (CRISPR base edited) This paper N/A MEF cells: wild type Vivek Garg Garg et al. (2021) MEF cells: MICU1-KO Vivek Garg Garg et al. (2021) (Continued on next page) e2 Molecular Cell 82, 3661–3676.e1–e8, October 6, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER MEF cells: MICU2-KO Vivek Garg Garg et al. (2021) Experimental models: Organisms/strains C57BL/6J mouse: wild type Jackson Laboratory Cat#000664; RRID: IMSR_JAX:000,664 Sprague-Dawley rats: wild type Charles River Laboratory Cat#001; RRID: RGD_734476 Oligonucleotides sgRNA sequence to mutate native MICU1 to C463R in HEK cells: 50-ACACCTTCCTGTGCACATTTCCACAG This paper N/A Recombinant DNA MCU, EMRE, MICU1 or MICU2 in pcDNA3.1(+) Home made Tsai et al. (2016); Tsai et al., 2017 MICU1 or MICU2 in pLJM1 This paper N/A pLKO.hygro constructs to knockdown YME1L1 or AFG3L2 Home made Tsai et al., 2017 pCMV_ABEmax Koblan et al. (2018) Addgene Cat112095 MLM3636 sgRNA expression plasmid Keith Joung Addgene Cat#43860 Mito-GCaMP6f Timothy Ryan Ashrafi et al. (2020) Cyto-GCaMP6f Chen et al. (2013) Addgene Cat#40755 Mito-Sypher Poburko et al. (2011) Addgene Cat#48251 Cyto-Sypher Poburko et al. (2011) Addgene Cat#48250 Mito-roGFP2-Orp1 Gutscher et al. (2009) Addgene Cat#64992 Mitochondria-targeted luciferase Paolo Pinton Morciano et al. (2017) Mito-GEM-GECO1 Zhao et al. (2011) Addgene Cat#32461 MBP-MICU1 in pET24a This paper N/A MICU2 in pETDuet This paper N/A Software and algorithms Igor pro (version 8) WaveMetrics www.wavemetrics.com/products/igorpro Image Studio (version 5.2) LI-COR https://www.licor.com/bio/image-studio/ ImageJ (Mac OS X) ImageJ website https://ImageJ.nih.gov/ij Time Series Analyzer plug-in (version 3) Balaji J https://ImageJ.nih.gov/ij/plugins/time- series.html ..

    Recombinant:

    Article Title: Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RIPA buffer Santa Cruz Cat#sc-24948 Intercept blocking buffer (TBS-based) LI-COR Cat#927-60001 PVDF membranes LI-COR Cat#926-31097 Protease inhibitor cocktail tablet Roche Cat#05892970001 TALON cobalt affinity resin Takara Cat#635504 CNBr-activated Sepharose 4B GE Healthcare Cat#17043001 Cycloheximide Sigma Cat#C7698 Oligomycin Cayman Cat#11342 Rotenone Cayman Cat#13995 Antimycin A Sigma Cat#A8674 Ionomycin Cayman Cat#11932 Carbachol Cayman Cat#114486 45Ca2+ radioisotope Perkin-Elmer Cat#NEZ013001MC 35S-methionine Perkin-Elmer Cat#NEG009A001MC Cysteine/methionine free medium Sigma Cat#D0422 Protein A beads Santa Cruz Cat#sc-2001 Thapsigargin Cayman Cat#10522 Digitonin Sigma-Aldrich Cat#D141 Calcium green 5N Life Technologies Cat#C3737 TMRM Invitrogen Cat#T668 FCCP Cayman Cat#15218 Doxorubicin Tokyo Chemical Industry Cat#D4193 Cisplatin Tokyo Chemical Industry Cat#D3371 Annexin V CF488A Biotium Cat#29083 Propidium Iodide Cayman Cat#14289 Hoescht 33,258 Cayman Cat#16756 Luciferin ThermoFisher Cat#PI88291 Critical commercial assays Seahorse XF Real-time ATP Rate Assay Kit Agilent Cat#103591-100 QuickChange II Kit Agilent Cat#200524 BCA assay kit Thermo Cat#23225 NativePAGE Sample Prep kit Invitrogen Cat#BN2008 NativePAGE Running Buffer kit Invitrogen Cat#BN2007 Deposited data Unprocessed images used in the figures This paper https://doi.org/10.17632/shrtrdfhyt.1 Experimental models: Cell lines HEK 293T: wild type ATCC Cat#CRL-11268; RRID: CVCL_1926 HeLa: wild type ATCC Cat#CCL-2; RRID: CVCL_0030 U2OS: wild type Cell Technologies Resource Center in the University of Colorado Anschutz Medical Campus https://medschool.cuanschutz.edu/ colorado-cancer-center/research/ shared-resources/cell-technologies/ cell-culture-services HEK 293T: MICU1-KO Home made Tsai et al. (2017) HEK 293T: MICU2-KO Home made Fan et al. (2020) HEK 293T: MICU1-MICU2-KO Home made Fan et al. (2020) HEK 239T: C463R-MICU1 (CRISPR base edited) This paper N/A MEF cells: wild type Vivek Garg Garg et al. (2021) MEF cells: MICU1-KO Vivek Garg Garg et al. (2021) (Continued on next page) e2 Molecular Cell 82, 3661–3676.e1–e8, October 6, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER MEF cells: MICU2-KO Vivek Garg Garg et al. (2021) Experimental models: Organisms/strains C57BL/6J mouse: wild type Jackson Laboratory Cat#000664; RRID: IMSR_JAX:000,664 Sprague-Dawley rats: wild type Charles River Laboratory Cat#001; RRID: RGD_734476 Oligonucleotides sgRNA sequence to mutate native MICU1 to C463R in HEK cells: 50-ACACCTTCCTGTGCACATTTCCACAG This paper N/A Recombinant DNA MCU, EMRE, MICU1 or MICU2 in pcDNA3.1(+) Home made Tsai et al. (2016); Tsai et al., 2017 MICU1 or MICU2 in pLJM1 This paper N/A pLKO.hygro constructs to knockdown YME1L1 or AFG3L2 Home made Tsai et al., 2017 pCMV_ABEmax Koblan et al. (2018) Addgene Cat112095 MLM3636 sgRNA expression plasmid Keith Joung Addgene Cat#43860 Mito-GCaMP6f Timothy Ryan Ashrafi et al. (2020) Cyto-GCaMP6f Chen et al. (2013) Addgene Cat#40755 Mito-Sypher Poburko et al. (2011) Addgene Cat#48251 Cyto-Sypher Poburko et al. (2011) Addgene Cat#48250 Mito-roGFP2-Orp1 Gutscher et al. (2009) Addgene Cat#64992 Mitochondria-targeted luciferase Paolo Pinton Morciano et al. (2017) Mito-GEM-GECO1 Zhao et al. (2011) Addgene Cat#32461 MBP-MICU1 in pET24a This paper N/A MICU2 in pETDuet This paper N/A Software and algorithms Igor pro (version 8) WaveMetrics www.wavemetrics.com/products/igorpro Image Studio (version 5.2) LI-COR https://www.licor.com/bio/image-studio/ ImageJ (Mac OS X) ImageJ website https://ImageJ.nih.gov/ij Time Series Analyzer plug-in (version 3) Balaji J https://ImageJ.nih.gov/ij/plugins/time- series.html ..

    Construct:

    Article Title: Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RIPA buffer Santa Cruz Cat#sc-24948 Intercept blocking buffer (TBS-based) LI-COR Cat#927-60001 PVDF membranes LI-COR Cat#926-31097 Protease inhibitor cocktail tablet Roche Cat#05892970001 TALON cobalt affinity resin Takara Cat#635504 CNBr-activated Sepharose 4B GE Healthcare Cat#17043001 Cycloheximide Sigma Cat#C7698 Oligomycin Cayman Cat#11342 Rotenone Cayman Cat#13995 Antimycin A Sigma Cat#A8674 Ionomycin Cayman Cat#11932 Carbachol Cayman Cat#114486 45Ca2+ radioisotope Perkin-Elmer Cat#NEZ013001MC 35S-methionine Perkin-Elmer Cat#NEG009A001MC Cysteine/methionine free medium Sigma Cat#D0422 Protein A beads Santa Cruz Cat#sc-2001 Thapsigargin Cayman Cat#10522 Digitonin Sigma-Aldrich Cat#D141 Calcium green 5N Life Technologies Cat#C3737 TMRM Invitrogen Cat#T668 FCCP Cayman Cat#15218 Doxorubicin Tokyo Chemical Industry Cat#D4193 Cisplatin Tokyo Chemical Industry Cat#D3371 Annexin V CF488A Biotium Cat#29083 Propidium Iodide Cayman Cat#14289 Hoescht 33,258 Cayman Cat#16756 Luciferin ThermoFisher Cat#PI88291 Critical commercial assays Seahorse XF Real-time ATP Rate Assay Kit Agilent Cat#103591-100 QuickChange II Kit Agilent Cat#200524 BCA assay kit Thermo Cat#23225 NativePAGE Sample Prep kit Invitrogen Cat#BN2008 NativePAGE Running Buffer kit Invitrogen Cat#BN2007 Deposited data Unprocessed images used in the figures This paper https://doi.org/10.17632/shrtrdfhyt.1 Experimental models: Cell lines HEK 293T: wild type ATCC Cat#CRL-11268; RRID: CVCL_1926 HeLa: wild type ATCC Cat#CCL-2; RRID: CVCL_0030 U2OS: wild type Cell Technologies Resource Center in the University of Colorado Anschutz Medical Campus https://medschool.cuanschutz.edu/ colorado-cancer-center/research/ shared-resources/cell-technologies/ cell-culture-services HEK 293T: MICU1-KO Home made Tsai et al. (2017) HEK 293T: MICU2-KO Home made Fan et al. (2020) HEK 293T: MICU1-MICU2-KO Home made Fan et al. (2020) HEK 239T: C463R-MICU1 (CRISPR base edited) This paper N/A MEF cells: wild type Vivek Garg Garg et al. (2021) MEF cells: MICU1-KO Vivek Garg Garg et al. (2021) (Continued on next page) e2 Molecular Cell 82, 3661–3676.e1–e8, October 6, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER MEF cells: MICU2-KO Vivek Garg Garg et al. (2021) Experimental models: Organisms/strains C57BL/6J mouse: wild type Jackson Laboratory Cat#000664; RRID: IMSR_JAX:000,664 Sprague-Dawley rats: wild type Charles River Laboratory Cat#001; RRID: RGD_734476 Oligonucleotides sgRNA sequence to mutate native MICU1 to C463R in HEK cells: 50-ACACCTTCCTGTGCACATTTCCACAG This paper N/A Recombinant DNA MCU, EMRE, MICU1 or MICU2 in pcDNA3.1(+) Home made Tsai et al. (2016); Tsai et al., 2017 MICU1 or MICU2 in pLJM1 This paper N/A pLKO.hygro constructs to knockdown YME1L1 or AFG3L2 Home made Tsai et al., 2017 pCMV_ABEmax Koblan et al. (2018) Addgene Cat112095 MLM3636 sgRNA expression plasmid Keith Joung Addgene Cat#43860 Mito-GCaMP6f Timothy Ryan Ashrafi et al. (2020) Cyto-GCaMP6f Chen et al. (2013) Addgene Cat#40755 Mito-Sypher Poburko et al. (2011) Addgene Cat#48251 Cyto-Sypher Poburko et al. (2011) Addgene Cat#48250 Mito-roGFP2-Orp1 Gutscher et al. (2009) Addgene Cat#64992 Mitochondria-targeted luciferase Paolo Pinton Morciano et al. (2017) Mito-GEM-GECO1 Zhao et al. (2011) Addgene Cat#32461 MBP-MICU1 in pET24a This paper N/A MICU2 in pETDuet This paper N/A Software and algorithms Igor pro (version 8) WaveMetrics www.wavemetrics.com/products/igorpro Image Studio (version 5.2) LI-COR https://www.licor.com/bio/image-studio/ ImageJ (Mac OS X) ImageJ website https://ImageJ.nih.gov/ij Time Series Analyzer plug-in (version 3) Balaji J https://ImageJ.nih.gov/ij/plugins/time- series.html ..

    Knockdown:

    Article Title: Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RIPA buffer Santa Cruz Cat#sc-24948 Intercept blocking buffer (TBS-based) LI-COR Cat#927-60001 PVDF membranes LI-COR Cat#926-31097 Protease inhibitor cocktail tablet Roche Cat#05892970001 TALON cobalt affinity resin Takara Cat#635504 CNBr-activated Sepharose 4B GE Healthcare Cat#17043001 Cycloheximide Sigma Cat#C7698 Oligomycin Cayman Cat#11342 Rotenone Cayman Cat#13995 Antimycin A Sigma Cat#A8674 Ionomycin Cayman Cat#11932 Carbachol Cayman Cat#114486 45Ca2+ radioisotope Perkin-Elmer Cat#NEZ013001MC 35S-methionine Perkin-Elmer Cat#NEG009A001MC Cysteine/methionine free medium Sigma Cat#D0422 Protein A beads Santa Cruz Cat#sc-2001 Thapsigargin Cayman Cat#10522 Digitonin Sigma-Aldrich Cat#D141 Calcium green 5N Life Technologies Cat#C3737 TMRM Invitrogen Cat#T668 FCCP Cayman Cat#15218 Doxorubicin Tokyo Chemical Industry Cat#D4193 Cisplatin Tokyo Chemical Industry Cat#D3371 Annexin V CF488A Biotium Cat#29083 Propidium Iodide Cayman Cat#14289 Hoescht 33,258 Cayman Cat#16756 Luciferin ThermoFisher Cat#PI88291 Critical commercial assays Seahorse XF Real-time ATP Rate Assay Kit Agilent Cat#103591-100 QuickChange II Kit Agilent Cat#200524 BCA assay kit Thermo Cat#23225 NativePAGE Sample Prep kit Invitrogen Cat#BN2008 NativePAGE Running Buffer kit Invitrogen Cat#BN2007 Deposited data Unprocessed images used in the figures This paper https://doi.org/10.17632/shrtrdfhyt.1 Experimental models: Cell lines HEK 293T: wild type ATCC Cat#CRL-11268; RRID: CVCL_1926 HeLa: wild type ATCC Cat#CCL-2; RRID: CVCL_0030 U2OS: wild type Cell Technologies Resource Center in the University of Colorado Anschutz Medical Campus https://medschool.cuanschutz.edu/ colorado-cancer-center/research/ shared-resources/cell-technologies/ cell-culture-services HEK 293T: MICU1-KO Home made Tsai et al. (2017) HEK 293T: MICU2-KO Home made Fan et al. (2020) HEK 293T: MICU1-MICU2-KO Home made Fan et al. (2020) HEK 239T: C463R-MICU1 (CRISPR base edited) This paper N/A MEF cells: wild type Vivek Garg Garg et al. (2021) MEF cells: MICU1-KO Vivek Garg Garg et al. (2021) (Continued on next page) e2 Molecular Cell 82, 3661–3676.e1–e8, October 6, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER MEF cells: MICU2-KO Vivek Garg Garg et al. (2021) Experimental models: Organisms/strains C57BL/6J mouse: wild type Jackson Laboratory Cat#000664; RRID: IMSR_JAX:000,664 Sprague-Dawley rats: wild type Charles River Laboratory Cat#001; RRID: RGD_734476 Oligonucleotides sgRNA sequence to mutate native MICU1 to C463R in HEK cells: 50-ACACCTTCCTGTGCACATTTCCACAG This paper N/A Recombinant DNA MCU, EMRE, MICU1 or MICU2 in pcDNA3.1(+) Home made Tsai et al. (2016); Tsai et al., 2017 MICU1 or MICU2 in pLJM1 This paper N/A pLKO.hygro constructs to knockdown YME1L1 or AFG3L2 Home made Tsai et al., 2017 pCMV_ABEmax Koblan et al. (2018) Addgene Cat112095 MLM3636 sgRNA expression plasmid Keith Joung Addgene Cat#43860 Mito-GCaMP6f Timothy Ryan Ashrafi et al. (2020) Cyto-GCaMP6f Chen et al. (2013) Addgene Cat#40755 Mito-Sypher Poburko et al. (2011) Addgene Cat#48251 Cyto-Sypher Poburko et al. (2011) Addgene Cat#48250 Mito-roGFP2-Orp1 Gutscher et al. (2009) Addgene Cat#64992 Mitochondria-targeted luciferase Paolo Pinton Morciano et al. (2017) Mito-GEM-GECO1 Zhao et al. (2011) Addgene Cat#32461 MBP-MICU1 in pET24a This paper N/A MICU2 in pETDuet This paper N/A Software and algorithms Igor pro (version 8) WaveMetrics www.wavemetrics.com/products/igorpro Image Studio (version 5.2) LI-COR https://www.licor.com/bio/image-studio/ ImageJ (Mac OS X) ImageJ website https://ImageJ.nih.gov/ij Time Series Analyzer plug-in (version 3) Balaji J https://ImageJ.nih.gov/ij/plugins/time- series.html ..

    Expressing:

    Article Title: Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RIPA buffer Santa Cruz Cat#sc-24948 Intercept blocking buffer (TBS-based) LI-COR Cat#927-60001 PVDF membranes LI-COR Cat#926-31097 Protease inhibitor cocktail tablet Roche Cat#05892970001 TALON cobalt affinity resin Takara Cat#635504 CNBr-activated Sepharose 4B GE Healthcare Cat#17043001 Cycloheximide Sigma Cat#C7698 Oligomycin Cayman Cat#11342 Rotenone Cayman Cat#13995 Antimycin A Sigma Cat#A8674 Ionomycin Cayman Cat#11932 Carbachol Cayman Cat#114486 45Ca2+ radioisotope Perkin-Elmer Cat#NEZ013001MC 35S-methionine Perkin-Elmer Cat#NEG009A001MC Cysteine/methionine free medium Sigma Cat#D0422 Protein A beads Santa Cruz Cat#sc-2001 Thapsigargin Cayman Cat#10522 Digitonin Sigma-Aldrich Cat#D141 Calcium green 5N Life Technologies Cat#C3737 TMRM Invitrogen Cat#T668 FCCP Cayman Cat#15218 Doxorubicin Tokyo Chemical Industry Cat#D4193 Cisplatin Tokyo Chemical Industry Cat#D3371 Annexin V CF488A Biotium Cat#29083 Propidium Iodide Cayman Cat#14289 Hoescht 33,258 Cayman Cat#16756 Luciferin ThermoFisher Cat#PI88291 Critical commercial assays Seahorse XF Real-time ATP Rate Assay Kit Agilent Cat#103591-100 QuickChange II Kit Agilent Cat#200524 BCA assay kit Thermo Cat#23225 NativePAGE Sample Prep kit Invitrogen Cat#BN2008 NativePAGE Running Buffer kit Invitrogen Cat#BN2007 Deposited data Unprocessed images used in the figures This paper https://doi.org/10.17632/shrtrdfhyt.1 Experimental models: Cell lines HEK 293T: wild type ATCC Cat#CRL-11268; RRID: CVCL_1926 HeLa: wild type ATCC Cat#CCL-2; RRID: CVCL_0030 U2OS: wild type Cell Technologies Resource Center in the University of Colorado Anschutz Medical Campus https://medschool.cuanschutz.edu/ colorado-cancer-center/research/ shared-resources/cell-technologies/ cell-culture-services HEK 293T: MICU1-KO Home made Tsai et al. (2017) HEK 293T: MICU2-KO Home made Fan et al. (2020) HEK 293T: MICU1-MICU2-KO Home made Fan et al. (2020) HEK 239T: C463R-MICU1 (CRISPR base edited) This paper N/A MEF cells: wild type Vivek Garg Garg et al. (2021) MEF cells: MICU1-KO Vivek Garg Garg et al. (2021) (Continued on next page) e2 Molecular Cell 82, 3661–3676.e1–e8, October 6, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER MEF cells: MICU2-KO Vivek Garg Garg et al. (2021) Experimental models: Organisms/strains C57BL/6J mouse: wild type Jackson Laboratory Cat#000664; RRID: IMSR_JAX:000,664 Sprague-Dawley rats: wild type Charles River Laboratory Cat#001; RRID: RGD_734476 Oligonucleotides sgRNA sequence to mutate native MICU1 to C463R in HEK cells: 50-ACACCTTCCTGTGCACATTTCCACAG This paper N/A Recombinant DNA MCU, EMRE, MICU1 or MICU2 in pcDNA3.1(+) Home made Tsai et al. (2016); Tsai et al., 2017 MICU1 or MICU2 in pLJM1 This paper N/A pLKO.hygro constructs to knockdown YME1L1 or AFG3L2 Home made Tsai et al., 2017 pCMV_ABEmax Koblan et al. (2018) Addgene Cat112095 MLM3636 sgRNA expression plasmid Keith Joung Addgene Cat#43860 Mito-GCaMP6f Timothy Ryan Ashrafi et al. (2020) Cyto-GCaMP6f Chen et al. (2013) Addgene Cat#40755 Mito-Sypher Poburko et al. (2011) Addgene Cat#48251 Cyto-Sypher Poburko et al. (2011) Addgene Cat#48250 Mito-roGFP2-Orp1 Gutscher et al. (2009) Addgene Cat#64992 Mitochondria-targeted luciferase Paolo Pinton Morciano et al. (2017) Mito-GEM-GECO1 Zhao et al. (2011) Addgene Cat#32461 MBP-MICU1 in pET24a This paper N/A MICU2 in pETDuet This paper N/A Software and algorithms Igor pro (version 8) WaveMetrics www.wavemetrics.com/products/igorpro Image Studio (version 5.2) LI-COR https://www.licor.com/bio/image-studio/ ImageJ (Mac OS X) ImageJ website https://ImageJ.nih.gov/ij Time Series Analyzer plug-in (version 3) Balaji J https://ImageJ.nih.gov/ij/plugins/time- series.html ..

    Plasmid Preparation:

    Article Title: Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RIPA buffer Santa Cruz Cat#sc-24948 Intercept blocking buffer (TBS-based) LI-COR Cat#927-60001 PVDF membranes LI-COR Cat#926-31097 Protease inhibitor cocktail tablet Roche Cat#05892970001 TALON cobalt affinity resin Takara Cat#635504 CNBr-activated Sepharose 4B GE Healthcare Cat#17043001 Cycloheximide Sigma Cat#C7698 Oligomycin Cayman Cat#11342 Rotenone Cayman Cat#13995 Antimycin A Sigma Cat#A8674 Ionomycin Cayman Cat#11932 Carbachol Cayman Cat#114486 45Ca2+ radioisotope Perkin-Elmer Cat#NEZ013001MC 35S-methionine Perkin-Elmer Cat#NEG009A001MC Cysteine/methionine free medium Sigma Cat#D0422 Protein A beads Santa Cruz Cat#sc-2001 Thapsigargin Cayman Cat#10522 Digitonin Sigma-Aldrich Cat#D141 Calcium green 5N Life Technologies Cat#C3737 TMRM Invitrogen Cat#T668 FCCP Cayman Cat#15218 Doxorubicin Tokyo Chemical Industry Cat#D4193 Cisplatin Tokyo Chemical Industry Cat#D3371 Annexin V CF488A Biotium Cat#29083 Propidium Iodide Cayman Cat#14289 Hoescht 33,258 Cayman Cat#16756 Luciferin ThermoFisher Cat#PI88291 Critical commercial assays Seahorse XF Real-time ATP Rate Assay Kit Agilent Cat#103591-100 QuickChange II Kit Agilent Cat#200524 BCA assay kit Thermo Cat#23225 NativePAGE Sample Prep kit Invitrogen Cat#BN2008 NativePAGE Running Buffer kit Invitrogen Cat#BN2007 Deposited data Unprocessed images used in the figures This paper https://doi.org/10.17632/shrtrdfhyt.1 Experimental models: Cell lines HEK 293T: wild type ATCC Cat#CRL-11268; RRID: CVCL_1926 HeLa: wild type ATCC Cat#CCL-2; RRID: CVCL_0030 U2OS: wild type Cell Technologies Resource Center in the University of Colorado Anschutz Medical Campus https://medschool.cuanschutz.edu/ colorado-cancer-center/research/ shared-resources/cell-technologies/ cell-culture-services HEK 293T: MICU1-KO Home made Tsai et al. (2017) HEK 293T: MICU2-KO Home made Fan et al. (2020) HEK 293T: MICU1-MICU2-KO Home made Fan et al. (2020) HEK 239T: C463R-MICU1 (CRISPR base edited) This paper N/A MEF cells: wild type Vivek Garg Garg et al. (2021) MEF cells: MICU1-KO Vivek Garg Garg et al. (2021) (Continued on next page) e2 Molecular Cell 82, 3661–3676.e1–e8, October 6, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER MEF cells: MICU2-KO Vivek Garg Garg et al. (2021) Experimental models: Organisms/strains C57BL/6J mouse: wild type Jackson Laboratory Cat#000664; RRID: IMSR_JAX:000,664 Sprague-Dawley rats: wild type Charles River Laboratory Cat#001; RRID: RGD_734476 Oligonucleotides sgRNA sequence to mutate native MICU1 to C463R in HEK cells: 50-ACACCTTCCTGTGCACATTTCCACAG This paper N/A Recombinant DNA MCU, EMRE, MICU1 or MICU2 in pcDNA3.1(+) Home made Tsai et al. (2016); Tsai et al., 2017 MICU1 or MICU2 in pLJM1 This paper N/A pLKO.hygro constructs to knockdown YME1L1 or AFG3L2 Home made Tsai et al., 2017 pCMV_ABEmax Koblan et al. (2018) Addgene Cat112095 MLM3636 sgRNA expression plasmid Keith Joung Addgene Cat#43860 Mito-GCaMP6f Timothy Ryan Ashrafi et al. (2020) Cyto-GCaMP6f Chen et al. (2013) Addgene Cat#40755 Mito-Sypher Poburko et al. (2011) Addgene Cat#48251 Cyto-Sypher Poburko et al. (2011) Addgene Cat#48250 Mito-roGFP2-Orp1 Gutscher et al. (2009) Addgene Cat#64992 Mitochondria-targeted luciferase Paolo Pinton Morciano et al. (2017) Mito-GEM-GECO1 Zhao et al. (2011) Addgene Cat#32461 MBP-MICU1 in pET24a This paper N/A MICU2 in pETDuet This paper N/A Software and algorithms Igor pro (version 8) WaveMetrics www.wavemetrics.com/products/igorpro Image Studio (version 5.2) LI-COR https://www.licor.com/bio/image-studio/ ImageJ (Mac OS X) ImageJ website https://ImageJ.nih.gov/ij Time Series Analyzer plug-in (version 3) Balaji J https://ImageJ.nih.gov/ij/plugins/time- series.html ..

    Luciferase:

    Article Title: Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RIPA buffer Santa Cruz Cat#sc-24948 Intercept blocking buffer (TBS-based) LI-COR Cat#927-60001 PVDF membranes LI-COR Cat#926-31097 Protease inhibitor cocktail tablet Roche Cat#05892970001 TALON cobalt affinity resin Takara Cat#635504 CNBr-activated Sepharose 4B GE Healthcare Cat#17043001 Cycloheximide Sigma Cat#C7698 Oligomycin Cayman Cat#11342 Rotenone Cayman Cat#13995 Antimycin A Sigma Cat#A8674 Ionomycin Cayman Cat#11932 Carbachol Cayman Cat#114486 45Ca2+ radioisotope Perkin-Elmer Cat#NEZ013001MC 35S-methionine Perkin-Elmer Cat#NEG009A001MC Cysteine/methionine free medium Sigma Cat#D0422 Protein A beads Santa Cruz Cat#sc-2001 Thapsigargin Cayman Cat#10522 Digitonin Sigma-Aldrich Cat#D141 Calcium green 5N Life Technologies Cat#C3737 TMRM Invitrogen Cat#T668 FCCP Cayman Cat#15218 Doxorubicin Tokyo Chemical Industry Cat#D4193 Cisplatin Tokyo Chemical Industry Cat#D3371 Annexin V CF488A Biotium Cat#29083 Propidium Iodide Cayman Cat#14289 Hoescht 33,258 Cayman Cat#16756 Luciferin ThermoFisher Cat#PI88291 Critical commercial assays Seahorse XF Real-time ATP Rate Assay Kit Agilent Cat#103591-100 QuickChange II Kit Agilent Cat#200524 BCA assay kit Thermo Cat#23225 NativePAGE Sample Prep kit Invitrogen Cat#BN2008 NativePAGE Running Buffer kit Invitrogen Cat#BN2007 Deposited data Unprocessed images used in the figures This paper https://doi.org/10.17632/shrtrdfhyt.1 Experimental models: Cell lines HEK 293T: wild type ATCC Cat#CRL-11268; RRID: CVCL_1926 HeLa: wild type ATCC Cat#CCL-2; RRID: CVCL_0030 U2OS: wild type Cell Technologies Resource Center in the University of Colorado Anschutz Medical Campus https://medschool.cuanschutz.edu/ colorado-cancer-center/research/ shared-resources/cell-technologies/ cell-culture-services HEK 293T: MICU1-KO Home made Tsai et al. (2017) HEK 293T: MICU2-KO Home made Fan et al. (2020) HEK 293T: MICU1-MICU2-KO Home made Fan et al. (2020) HEK 239T: C463R-MICU1 (CRISPR base edited) This paper N/A MEF cells: wild type Vivek Garg Garg et al. (2021) MEF cells: MICU1-KO Vivek Garg Garg et al. (2021) (Continued on next page) e2 Molecular Cell 82, 3661–3676.e1–e8, October 6, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER MEF cells: MICU2-KO Vivek Garg Garg et al. (2021) Experimental models: Organisms/strains C57BL/6J mouse: wild type Jackson Laboratory Cat#000664; RRID: IMSR_JAX:000,664 Sprague-Dawley rats: wild type Charles River Laboratory Cat#001; RRID: RGD_734476 Oligonucleotides sgRNA sequence to mutate native MICU1 to C463R in HEK cells: 50-ACACCTTCCTGTGCACATTTCCACAG This paper N/A Recombinant DNA MCU, EMRE, MICU1 or MICU2 in pcDNA3.1(+) Home made Tsai et al. (2016); Tsai et al., 2017 MICU1 or MICU2 in pLJM1 This paper N/A pLKO.hygro constructs to knockdown YME1L1 or AFG3L2 Home made Tsai et al., 2017 pCMV_ABEmax Koblan et al. (2018) Addgene Cat112095 MLM3636 sgRNA expression plasmid Keith Joung Addgene Cat#43860 Mito-GCaMP6f Timothy Ryan Ashrafi et al. (2020) Cyto-GCaMP6f Chen et al. (2013) Addgene Cat#40755 Mito-Sypher Poburko et al. (2011) Addgene Cat#48251 Cyto-Sypher Poburko et al. (2011) Addgene Cat#48250 Mito-roGFP2-Orp1 Gutscher et al. (2009) Addgene Cat#64992 Mitochondria-targeted luciferase Paolo Pinton Morciano et al. (2017) Mito-GEM-GECO1 Zhao et al. (2011) Addgene Cat#32461 MBP-MICU1 in pET24a This paper N/A MICU2 in pETDuet This paper N/A Software and algorithms Igor pro (version 8) WaveMetrics www.wavemetrics.com/products/igorpro Image Studio (version 5.2) LI-COR https://www.licor.com/bio/image-studio/ ImageJ (Mac OS X) ImageJ website https://ImageJ.nih.gov/ij Time Series Analyzer plug-in (version 3) Balaji J https://ImageJ.nih.gov/ij/plugins/time- series.html ..

    Software:

    Article Title: Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RIPA buffer Santa Cruz Cat#sc-24948 Intercept blocking buffer (TBS-based) LI-COR Cat#927-60001 PVDF membranes LI-COR Cat#926-31097 Protease inhibitor cocktail tablet Roche Cat#05892970001 TALON cobalt affinity resin Takara Cat#635504 CNBr-activated Sepharose 4B GE Healthcare Cat#17043001 Cycloheximide Sigma Cat#C7698 Oligomycin Cayman Cat#11342 Rotenone Cayman Cat#13995 Antimycin A Sigma Cat#A8674 Ionomycin Cayman Cat#11932 Carbachol Cayman Cat#114486 45Ca2+ radioisotope Perkin-Elmer Cat#NEZ013001MC 35S-methionine Perkin-Elmer Cat#NEG009A001MC Cysteine/methionine free medium Sigma Cat#D0422 Protein A beads Santa Cruz Cat#sc-2001 Thapsigargin Cayman Cat#10522 Digitonin Sigma-Aldrich Cat#D141 Calcium green 5N Life Technologies Cat#C3737 TMRM Invitrogen Cat#T668 FCCP Cayman Cat#15218 Doxorubicin Tokyo Chemical Industry Cat#D4193 Cisplatin Tokyo Chemical Industry Cat#D3371 Annexin V CF488A Biotium Cat#29083 Propidium Iodide Cayman Cat#14289 Hoescht 33,258 Cayman Cat#16756 Luciferin ThermoFisher Cat#PI88291 Critical commercial assays Seahorse XF Real-time ATP Rate Assay Kit Agilent Cat#103591-100 QuickChange II Kit Agilent Cat#200524 BCA assay kit Thermo Cat#23225 NativePAGE Sample Prep kit Invitrogen Cat#BN2008 NativePAGE Running Buffer kit Invitrogen Cat#BN2007 Deposited data Unprocessed images used in the figures This paper https://doi.org/10.17632/shrtrdfhyt.1 Experimental models: Cell lines HEK 293T: wild type ATCC Cat#CRL-11268; RRID: CVCL_1926 HeLa: wild type ATCC Cat#CCL-2; RRID: CVCL_0030 U2OS: wild type Cell Technologies Resource Center in the University of Colorado Anschutz Medical Campus https://medschool.cuanschutz.edu/ colorado-cancer-center/research/ shared-resources/cell-technologies/ cell-culture-services HEK 293T: MICU1-KO Home made Tsai et al. (2017) HEK 293T: MICU2-KO Home made Fan et al. (2020) HEK 293T: MICU1-MICU2-KO Home made Fan et al. (2020) HEK 239T: C463R-MICU1 (CRISPR base edited) This paper N/A MEF cells: wild type Vivek Garg Garg et al. (2021) MEF cells: MICU1-KO Vivek Garg Garg et al. (2021) (Continued on next page) e2 Molecular Cell 82, 3661–3676.e1–e8, October 6, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER MEF cells: MICU2-KO Vivek Garg Garg et al. (2021) Experimental models: Organisms/strains C57BL/6J mouse: wild type Jackson Laboratory Cat#000664; RRID: IMSR_JAX:000,664 Sprague-Dawley rats: wild type Charles River Laboratory Cat#001; RRID: RGD_734476 Oligonucleotides sgRNA sequence to mutate native MICU1 to C463R in HEK cells: 50-ACACCTTCCTGTGCACATTTCCACAG This paper N/A Recombinant DNA MCU, EMRE, MICU1 or MICU2 in pcDNA3.1(+) Home made Tsai et al. (2016); Tsai et al., 2017 MICU1 or MICU2 in pLJM1 This paper N/A pLKO.hygro constructs to knockdown YME1L1 or AFG3L2 Home made Tsai et al., 2017 pCMV_ABEmax Koblan et al. (2018) Addgene Cat112095 MLM3636 sgRNA expression plasmid Keith Joung Addgene Cat#43860 Mito-GCaMP6f Timothy Ryan Ashrafi et al. (2020) Cyto-GCaMP6f Chen et al. (2013) Addgene Cat#40755 Mito-Sypher Poburko et al. (2011) Addgene Cat#48251 Cyto-Sypher Poburko et al. (2011) Addgene Cat#48250 Mito-roGFP2-Orp1 Gutscher et al. (2009) Addgene Cat#64992 Mitochondria-targeted luciferase Paolo Pinton Morciano et al. (2017) Mito-GEM-GECO1 Zhao et al. (2011) Addgene Cat#32461 MBP-MICU1 in pET24a This paper N/A MICU2 in pETDuet This paper N/A Software and algorithms Igor pro (version 8) WaveMetrics www.wavemetrics.com/products/igorpro Image Studio (version 5.2) LI-COR https://www.licor.com/bio/image-studio/ ImageJ (Mac OS X) ImageJ website https://ImageJ.nih.gov/ij Time Series Analyzer plug-in (version 3) Balaji J https://ImageJ.nih.gov/ij/plugins/time- series.html ..



    Similar Products

    2017 pcmv abemax koblan  (Addgene inc)
    94
    Addgene inc 2017 pcmv abemax koblan
    2017 Pcmv Abemax Koblan, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2017+pcmv+abemax+koblan/pCMV_ABEmax+(Plasmid+%23112095)/pm36206740-308-91-97
    Average 94 stars, based on 1 article reviews
    2017 pcmv abemax koblan - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier

    Image Search Results